A general Ca-MOM platform with enhanced acid-base stability for enzyme biocatalysis

Whey upgrading by enzyme biocatalysis

Whey is a co-product of processes for the production of cheese and casein that retains most of the lactose content in milk. World production of whey is estimated around 200 million tons per year with an increase rate of about 2%/per year. Milk production is seasonal, so surplus whey is unavoidable. Traditionally, whey producers have considered it as a nuisance and strategies of whey handling ha...

Regeneration of Cofactors for Enzyme Biocatalysis

Acid-base Interactions 10.1 GENERAL CONCEPT OF ACID-BASE INTERACTIONS

Acid-base interactions have found numerous applications in research dealing with adsorption of molecules of liquids on the surfaces of solids. The main focus of this research is to estimate the thermodynamic work of adhesion, determine mechanism of interactions, analyze the morphology of interfaces and various surface coatings, develop surface modifiers, study the aggregation of macromolecular ...

Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration.

Although there have been a long history of studying and using immobilized enzymes, little has been reported regarding the nature of immobilized cofactors. Herein we report that cofactor NAD(H) covalently attached to silica nanoparticles successfully coordinated with particle-immobilized enzymes and enabled multistep biotransformations. Specifically, silica nanoparticle-attached glutamate dehydr...

Extreme Thermophiles: Moving beyond single-enzyme biocatalysis.

Extremely thermophilic microorganisms have been sources of thermostable and thermoactive enzymes for over 30 years. However, information and insights gained from genome sequences, in conjunction with new tools for molecular genetics, have opened up exciting new possibilities for biotechnological opportunities based on extreme thermophiles that go beyond single-step biotransformations. Although ...